Dystrophin Monoklonaler Antikörper

Dystrophin Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G12E6

Kat-Nr. : 68120-1-PBS

Synonyme

DMD, 1G12E6, BMD

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  WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68120-1-Ig (same clone as 68120-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68120-1-Ig (same clone as 68120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat heart using 68120-1-Ig (same clone as 68120-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 68120-1-Ig (same clone as 68120-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 68120-1-Ig (same clone as 68120-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68120-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68120-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA Dystrophin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Dystrophin fusion protein Ag4392
• Vollständiger Name: dystrophin
• Berechnetes Molekulargewicht: 3685 aa, 427 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 430 kDa
• GenBank-Zugangsnummer: BC028720
• Gene symbol: Dystrophin
• Gene ID (NCBI): 1756
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.