Dystrophin Rekombinanter Antikörper

Dystrophin Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240567A3

Kat-Nr. : 83609-5-PBS

Synonyme

DMD, 240567A3, BMD

---

• 

  WB analysis using 83609-5-RR (same clone as 83609-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83609-5-RR (DMD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 83609-5-RR (same clone as 83609-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83609-5-RR (Dystrophin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 83609-5-RR (same clone as 83609-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 83609-5-RR (same clone as 83609-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 83609-5-RR (same clone as 83609-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CoraLite® Plus 488 ACTN2 antibody (CL488-14221, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  IF Staining of rat heart using 83609-5-RR (same clone as 83609-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha cardiac muscle actin specific antibody (66125-1-Ig, Clone: 1F2B9, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83609-5-RR (same clone as 83609-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83609-5-RR (same clone as 83609-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83609-5-RR (same clone as 83609-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Dystrophin Recombinant antibody (83609-5-RR, Clone:240567A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83609-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83609-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83609-5-RR against Human Dystrophin were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

83609-5-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Dystrophin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Dystrophin fusion protein Ag3408
• Vollständiger Name: dystrophin
• Berechnetes Molekulargewicht: 3685 aa, 427 kDa
• Beobachtetes Molekulargewicht: 430 kDa, 70 kDa
• GenBank-Zugangsnummer: BC028720
• Gene symbol: Dystrophin
• Gene ID (NCBI): 1756
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.