E-Cadherin Polyklonaler Antikörper

E-Cadherin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31863-1-AP

Synonyme

cadherin 1, Cdh1, Ecad, L CAM, Um

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  WB analysis using 31863-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31863-1-AP (E-Cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 31863-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31863-1-AP (E-Cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 31863-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31863-1-AP (E-Cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 31863-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31863-1-AP (E-Cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 31863-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31863-1-AP (E-Cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 31863-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-Cadherin antibody (31863-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, 4T1-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31863-1-AP bindet in WB, IHC, IF/ICC, ELISA E-Cadherin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: E-Cadherin fusion protein Eg0975
• Vollständiger Name: cadherin 1
• Berechnetes Molekulargewicht: 98kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_009864.3
• Gene symbol: E-cadherin
• Gene ID (NCBI): 12550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.