E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241282A5

Kat-Nr. : 84111-1-PBS

Synonyme

cadherin 1, Cdh1, Ecad, L CAM, Um

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  Sandwich ELISA sample test of MP01047-2

  The mean E-Cadherin concentration was determined to be 10.6 ng/mL in mouse liver tissue extract based on a 5.0 mg/mL extract load.

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  Sandwich ELISA sample test of MP01047-2

  Serum of six mice was measured. The E-Cadherin concentration of detected samples was determined to be 189.4 ng/mL with a range of 166.7-209.1 ng/mL.

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  Sandwich ELISA standard curve of MP01047-2

  Sandwich ELISA standard curve of MP01047-2, Mouse E-Cadherin Recombinant Matched Antibody Pair - PBS only. 84111-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0975. 84111-2-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

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  IHC staining of mouse colon using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 84111-1-RR (same clone as 84111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01047-2

  Cytometric bead array standard curve of MP01047-2, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-1-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84111-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84111-1-RR against Mouse E-cadherin were performed. The affinity constant is 0.519 nM.

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Geprüfte Anwendungen

Produktinformation

84111-1-PBS bindet in IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA E-cadherin und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Eg0975
• Vollständiger Name: cadherin 1
• Berechnetes Molekulargewicht: 98kDa
• GenBank-Zugangsnummer: NM_009864.3
• Gene symbol: E-cadherin
• Gene ID (NCBI): 12550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.