E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241282A5

Kat-Nr. : 84111-1-RR

Synonyme

cadherin 1, Cdh1, Ecad, L CAM, Um

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  IHC staining of mouse colon using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 84111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84111-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84111-1-RR against Mouse E-cadherin were performed. The affinity constant is 0.519 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, Mausnierengewebe, Maushautgewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84111-1-RR bindet in IHC, ELISA E-cadherin und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Eg0975
• Vollständiger Name: cadherin 1
• Berechnetes Molekulargewicht: 98kDa
• GenBank-Zugangsnummer: NM_009864.3
• Gene symbol: E-cadherin
• Gene ID (NCBI): 12550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.