E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242018D3

Kat-Nr. : 84646-3-PBS

Synonyme

242018D3, Cadherin-1, CD324, Cdh1, E-Cad/CTF1

---

• 

  WB analysis using 84646-3-RR (same clone as 84646-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84646-3-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01483-1

  Sandwich ELISA standard curve of MP01483-1, Rat E-Cadherin Recombinant Matched Antibody Pair - PBS only. 84646-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1229. 84646-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01483-1

  Serum of six rats was measured. The E-Cadherin concentration of detected samples was determined to be 673.3 ng/mL with a range of 608.2-748.3 ng/mL.

• 

  Sample test of MP01483-1

  Urine of six rats was measured. The E-Cadherin concentration of detected samples was determined to be 5,367.9 ng/mL with a range of 1,627.0-10,091.3 ng/mL.

• 

  Sample test of MP01483-1

  The mean E-Cadherin concentration was determined to be 33.3 ng/mL in rat spleen tissue extract based on a 2.7 mg/mL extract load.

• 

  IHC staining of rat colon using 84646-3-RR (same clone as 84646-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 84646-3-RR (same clone as 84646-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

• 

  IF Staining of rat colon using 84646-3-RR (same clone as 84646-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using E-Cadherin antibody (84646-3-RR, Clone: 242018D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 84646-3-RR (same clone as 84646-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-Cadherin antibody (84646-3-RR, Clone: 242018D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01483-1

  Cytometric bead array standard curve of MP01483-1, RAT E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84646-1-PBS. Detection antibody: 84646-3-PBS. Standard: Eg1229. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84646-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84646-3-RR against Rat E-Cadherin were performed. The affinity constant is 1.84 nM.

---

Geprüfte Anwendungen

Produktinformation

84646-3-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test E-cadherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Eg1229
• Vollständiger Name: cadherin 1
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: NM_031334.1
• Gene symbol: Cdh1
• Gene ID (NCBI): 83502
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.