E-cadherin Polyklonaler Antikörper

E-cadherin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 31515-1-AP

Synonyme

CDH1, CD324, CAM 120/80, Cadherin-1, Cadherin 1

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  WB analysis using 31515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31515-1-AP (E-cadherin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31515-1-AP (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 31515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31515-1-AP (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 31515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31515-1-AP (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 31515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31515-1-AP (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 31515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31515-1-AP (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 31515-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31515-1-AP (E-cadherin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 31515-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (31515-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, MCF-7-Zellen, Maushautgewebe, Ratten-Kolongewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: Maushautgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

31515-1-AP bindet in WB, IHC, IF/ICC, ELISA E-cadherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: cadherin 1, type 1, E-cadherin (epithelial)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 120-125 kDa, 80-90 kDa
• GenBank-Zugangsnummer: NM_004360.5
• Gene symbol: E-cadherin
• Gene ID (NCBI): 999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	PLoS Pathog; Lactobacillus gasseri ATCC33323 affects the intestinal mucosal barrier to ameliorate DSS-induced colitis through the NR1I3-mediated regulation of E-cadherin; Authors - Guanru Qian
human	WB	Nutrients; Methanolic Extract of Cimicifuga foetida Induces G1 Cell Cycle Arrest and Apoptosis and Inhibits Metastasis of Glioma Cells; Authors - Chih-Hsuan Chang
human	WB	Sci Rep; N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer; Authors - Fuqi Wang
mouse	WB	Front Med (Lausanne); PCSK9 inhibitor alleviates experimental pulmonary fibrosis-induced pulmonary hypertension via attenuating epithelial-mesenchymal transition by suppressing Wnt/β-catenin signaling in vivo and in vitro; Authors - Jiancheng Lin
human	WB	Biomater Res; An In Situ Sustained-Release Chitosan Hydrogel to Attenuate Renal Fibrosis by Retaining Klotho Expression; Authors - Chenyang Li
human	WB	J Cancer; HULC-IGF2BP2 Interaction Drives Proliferation and Metastasis in Colorectal Cancer; Authors - Qiang Pang