E-cadherin Monoklonaler Antikörper

E-cadherin Monoklonal Antikörper für IF-P, FC

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

human, Hund, Maus

Anwendung

IF-P, FC

Konjugation

Unkonjugiert

CloneNo.

DECMA-1

Kat-Nr. : 65241-1-Ig

Synonyme

CD324 (E-cadherin), cadherin 1, Cdh1, Ecad, L CAM



Geprüfte Anwendungen

Erfolgreiche Detektion in IF-PMaus-Kolongewebe
Erfolgreiche Detektion in FCMDCK-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)-PIF-P : 1:625-1:2500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65241-1-Ig bindet in IF-P, FC E-cadherin und zeigt Reaktivität mit human, Hund, Maus

Getestete Reaktivität human, Hund, Maus
Wirt / Isotyp Ratte / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Mouse embryonal carcinoma cell line PCC4 Aza RI
Vollständiger Name cadherin 1
GenBank-ZugangsnummerBC098501
Gene symbol E-cadherin
Gene ID (NCBI) 12550
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide
LagerungsbedingungenStore at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for E-cadherin antibody 65241-1-IgProtokoll herunterladen
FC protocol for E-cadherin antibody 65241-1-IgDownload protocol
STANDARD-PROTOKOLLE
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