E-cadherin Monoklonaler Antikörper

E-cadherin Monoklonal Antikörper für IF-P, FC

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

human, Hund, Maus

Anwendung

IF-P, FC

Konjugation

Unkonjugiert

CloneNo.

DECMA-1

Kat-Nr. : 65241-1-Ig

Synonyme

CD324 (E-cadherin), cadherin 1, Cdh1, Ecad, L CAM

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  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MDCK using 65241-1-Ig

  1X10^6 MDCK cells were surface stained with 0.2 ug Anti-Mouse CD324 (E-cadherin) (65241-1-Ig, Clone:DECMA-1) and APC-conjugated Goat Anti-Rat IgG at dilution 1:1000. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Kolongewebe
• Erfolgreiche Detektion in FC: MDCK-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:625-1:2500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65241-1-Ig bindet in IF-P, FC E-cadherin und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Ratte / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Mouse embryonal carcinoma cell line PCC4 Aza RI
• Vollständiger Name: cadherin 1
• GenBank-Zugangsnummer: BC098501
• Gene symbol: E-cadherin
• Gene ID (NCBI): 12550
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.