E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

240159C10

Kat-Nr. : 80541-5-RR

Synonyme

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHCT 116-Zellen, A431-Zellen, Caco-2-Zellen, MCF-7-Zellen, T-47D-Zellen
Erfolgreiche Detektion in IHChumanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 6 publications below
IFSee 1 publications below

Produktinformation

80541-5-RR bindet in WB, IHC, IF/ICC, ELISA E-cadherin und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen E-cadherin fusion protein Ag14973
Vollständiger Name cadherin 1, type 1, E-cadherin (epithelial)
Berechnetes Molekulargewicht 882 aa, 97 kDa
Beobachtetes Molekulargewicht135 kDa
GenBank-ZugangsnummerBC141838
Gene symbol E-cadherin
Gene ID (NCBI) 999
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for E-cadherin antibody 80541-5-RRProtokoll herunterladen
IHC protocol for E-cadherin antibody 80541-5-RRProtokoll herunterladenl
IF protocol for E-cadherin antibody 80541-5-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Sci Rep

Hypoxia and lipid metabolism related genes drive proliferation migration and immune infiltration mechanisms in colorectal cancer subtyping

Authors - Shansong Huang
humanWB

J Cancer Res Clin Oncol

CircWDR78 inhibits the development of colorectal cancer by regulating the miR-653-3p/RGS4 axis

Authors - Chu Hao
mouseWB,IF

Sci Rep

Molecular mechanisms of lung injury from ultra high and conventional dose rate pulsed radiation based on 4D DIA proteomics study

Authors - MengHua Li
humanWB

Int Immunopharmacol

Role of COL5A1 in lung squamous cell Carcinoma: Prognostic Implications and therapeutic potential

Authors - Chengjuan Zhang
humanWB

Pharmaceuticals (Basel)

Antitumor Activity of USP7 Inhibitor GNE-6776 in Non-Small Cell Lung Cancer Involves Regulation of Epithelial-Mesenchymal Transition, Cell Cycle, Wnt/β-Catenin, and PI3K/AKT/mTOR Pathways

Authors - Lipeng Wu
humanWB

Nat Commun

Splicing diversity enhances the molecular classification of pituitary neuroendocrine tumors

Authors - Yue Huang