E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240159G8

Kat-Nr. : 80541-9-RR

Synonyme

240159G8, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

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  WB analysis using 80541-9-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80541-9-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80541-9-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80541-9-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 80541-9-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80541-9-RR (E-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 80541-9-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80541-9-RR (E-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon using 80541-9-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using E-cadherin antibody (80541-9-RR, Clone: 240159G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon using 80541-9-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using E-cadherin antibody (80541-9-RR, Clone: 240159G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 80541-9-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-9-RR, Clone: 240159G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  Affinity and Kinetic Characterization of 80541-9-RR

  Biolayer interferometry (BLl) kinetic assays of 80541-9-RR against Human E-cadherin were performed. The affinity constant is 0.213 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, A431-Zellen, A549-Zellen, Caco-2-Zellen, HepG2-Zellen, MCF-7-Zellen, MKN-45-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: human colon tissue
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80541-9-RR bindet in WB, IHC, IF/ICC, IF-P, ELISA E-cadherin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Ag14973
• Vollständiger Name: cadherin 1, type 1, E-cadherin (epithelial)
• Berechnetes Molekulargewicht: 882 aa, 97 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC141838
• Gene symbol: E-cadherin
• Gene ID (NCBI): 999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.