E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240159G8

Kat-Nr. : 98030-1-RR

Synonyme

CD324 (E-cadherin), 240159G8, Arc 1, Cadherin 1, Cadherin-1

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  FC experiment of MCF-7 using 98030-1-RR

  1x10^6 MCF-7 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone: 240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of HT-29 using 98030-1-RR

  1x10^6 HT-29 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98030-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98030-1-RR against Human E-cadherin were performed. The affinity constant is 37.3 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: MCF-7-Zellen, HT-29-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98030-1-RR bindet in FC E-cadherin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Ag14973
• Vollständiger Name: cadherin 1, type 1, E-cadherin (epithelial)
• Berechnetes Molekulargewicht: 882 aa, 97 kDa
• GenBank-Zugangsnummer: BC141838
• Gene symbol: E-cadherin
• Gene ID (NCBI): 999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.