E-cadherin Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

E-cadherin Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6B11F11

Kat-Nr. : CL488-60335

Synonyme

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

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  IF Staining of human breast cancer using CL488-60335

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 E-cadherin antibody (CL488-60335, Clone: 6B11F11 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL488-60335

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 E-cadherin antibody (CL488-60335, Clone: 6B11F11 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL488-60335

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 E-cadherin antibody (CL488-60335, Clone: 6B11F11 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60335 bindet in IF/ICC, IF-P E-cadherin und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Ag15085
• Vollständiger Name: cadherin 1, type 1, E-cadherin (epithelial)
• Berechnetes Molekulargewicht: 882 aa, 97 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC141838
• Gene symbol: E-cadherin
• Gene ID (NCBI): 999
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.