- Featured Product
- KD/KO Validated
E-cadherin Monoklonaler Antikörper
E-cadherin Monoklonal Antikörper für IF/ICC, IF-Fro
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
Hausschwein, human, Maus
Anwendung
IF/ICC, IF-Fro
Konjugation
CoraLite®555 Fluorescent Dye
CloneNo.
6B11F11
Kat-Nr. : CL555-60335
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF-Fro | Maus-Dünndarmgewebe | 
| Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Immunfluoreszenz (IF)-FRO | IF-FRO : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL555-60335 bindet in IF/ICC, IF-Fro E-cadherin und zeigt Reaktivität mit Hausschwein, human, Maus
| Getestete Reaktivität | Hausschwein, human, Maus | 
| Wirt / Isotyp | Maus / IgG2b | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | E-cadherin fusion protein Ag15085 | 
| Vollständiger Name | cadherin 1, type 1, E-cadherin (epithelial) | 
| Berechnetes Molekulargewicht | 882 aa, 97 kDa | 
| Beobachtetes Molekulargewicht | 120 kDa | 
| GenBank-Zugangsnummer | BC141838 | 
| Gene symbol | E-cadherin | 
| Gene ID (NCBI) | 999 | 
| Konjugation | CoraLite®555 Fluorescent Dye | 
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm | 
| Form | Liquid | 
| Reinigungsmethode | Protein-A-Reinigung | 
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA | 
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IF protocol for CL555 E-cadherin antibody CL555-60335 | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 

