E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hund, Maus

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

241282C1

Kat-Nr. : CL594-98140

Synonyme

241282C1, cadherin 1, Cdh1, Ecad, L CAM

---

• 

  FC experiment of MDCK using CL594-98140

  1x10^6 MDCK cells were surface stained with 0.25 ug CoraLite®594 Anti-Mouse E-cadherin Rabbit RecAb (CL594-98140, Clone: 241282C1) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: MDCK-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98140 bindet in FC E-cadherin und zeigt Reaktivität mit Hund, Maus

• Getestete Reaktivität: Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Eg0975
• Vollständiger Name: cadherin 1
• Berechnetes Molekulargewicht: 98kDa
• GenBank-Zugangsnummer: NM_009864.3
• Gene symbol: E-cadherin
• Gene ID (NCBI): 12550
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.