E-selectin / CD62E Polyklonaler Antikörper

E-selectin / CD62E Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30205-1-AP

Synonyme

CD62E, E selectin, ELAM, ELAM 1, ELAM1, ESEL, LECAM2, SELE, selectin E

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  IHC staining of human appendicitis using 30205-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 30205-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 30205-1-AP (E-selectin / CD62E antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human appendicitis using 30205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human appendicitis using 30205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using E-selectin / CD62E antibody (30205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Appendizitis-Gewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30205-1-AP bindet in IHC, IF-P, ELISA E-selectin / CD62E und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: E-selectin / CD62E fusion protein Ag31820
• Vollständiger Name: selectin E
• Berechnetes Molekulargewicht: 610 aa, 67 kDa
• GenBank-Zugangsnummer: BC142711
• Gene symbol: CD62E
• Gene ID (NCBI): 6401
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)