E-selectin/CD62E Rekombinanter Antikörper

E-selectin/CD62E Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240700D4

Kat-Nr. : 98065-1-PBS

Synonyme

CD62E, E-selectin, 240700D4, E selectin, Elam

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  FC experiment of bEnd.3 using 98065-1-RR (same clone as 98065-1-PBS)

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse E-selectin/CD62E Rabbit Recombinant Antibody (98065-1-RR, Clone:240700D4) (red) or isotype control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98065-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98065-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98065-1-RR against Mouse E-selectin/CD62E were performed. The affinity constant is 4.40 nM.

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Geprüfte Anwendungen

Produktinformation

98065-1-PBS bindet in FC E-selectin/CD62E und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: selectin, endothelial cell
• Berechnetes Molekulargewicht: 67KD
• GenBank-Zugangsnummer: NM_011345.2
• Gene symbol: Sele
• Gene ID (NCBI): 20339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)