E-selectin/CD62E Rekombinanter Antikörper

E-selectin/CD62E Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240700D4

Kat-Nr. : 98065-1-RR

Synonyme

CD62E, E-selectin, 240700D4, E selectin, Elam

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  FC experiment of bEnd.3 using 98065-1-RR

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse E-selectin/CD62E Rabbit Recombinant Antibody (98065-1-RR, Clone:240700D4) (red) or isotype control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98065-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98065-1-RR against Mouse E-selectin/CD62E were performed. The affinity constant is 4.40 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: bEnd.3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98065-1-RR bindet in FC E-selectin/CD62E und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: selectin, endothelial cell
• Berechnetes Molekulargewicht: 67KD
• GenBank-Zugangsnummer: NM_011345.2
• Gene symbol: Sele
• Gene ID (NCBI): 20339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)