E-selectin/CD62E Rekombinanter Antikörper

E-selectin/CD62E Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241767H6

Kat-Nr. : CL647-98189

Synonyme

CD62E, E-selectin, 241767H6, CD62 antigen-like family member E, E selectin

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  FC experiment of HUVEC using CL647-98189

  1x10^6 untreated or TNF alpha-treated HUVEC cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human E-selectin/CD62E Rabbit RecAb (CL647-98189, Clone: 241767H6). Cells were not fixed.

• 

  FC experiment of HUVEC using CL647-98189

  1x10^6 TNF alpha-treated HUVEC cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human E-selectin/CD62E Rabbit RecAb (CL647-98189, Clone: 241767H6) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated HUVEC cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human E-selectin/CD62E Rabbit RecAb (CL647-98189, Clone:241767H6) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: TNF alpha treated HUVEC cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98189 bindet in FC E-selectin/CD62E und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: selectin E
• Berechnetes Molekulargewicht: 610 aa, 67 kDa
• GenBank-Zugangsnummer: BC142711
• Gene symbol: CD62E
• Gene ID (NCBI): 6401
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)