• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

E2F1 Monoklonaler Antikörper

E2F1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5D7G8

Kat-Nr. : 66515-1-PBS

Synonyme

5D7G8, E2F 1, E2F-1, pRB-binding protein E2F-1, RBAP-1

Filter:
  • Western Blot (WB) analysis of various lysates using E2F1 Monoclonal antibody (66515-1-Ig)

    WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using E2F1 Monoclonal antibody (66515-1-Ig)

    WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66515-1-PBS bindet in WB, Indirect ELISA E2F1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen E2F1 fusion protein Ag17363
Vollständiger Name E2F transcription factor 1
Berechnetes Molekulargewicht 437 aa, 47 kDa
Beobachtetes Molekulargewicht 55-60 kDa
GenBank-ZugangsnummerBC050369
Gene symbol E2F1
Gene ID (NCBI) 1869
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.