E2F8 Polyklonaler Antikörper

E2F8 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13425-1-AP

Synonyme

E2F 8, E2F transcription factor 8, E2F8, Transcription factor E2F8

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13425-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13425-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13425-1-AP

  IP Result of anti-E2F8 (IP:13425-1-AP, 4ug; Detection:13425-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• ChIP: See 2 publications below

Produktinformation

13425-1-AP bindet in WB, IP, ChIP, ELISA E2F8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: E2F8 fusion protein Ag4216
• Vollständiger Name: E2F transcription factor 8
• Berechnetes Molekulargewicht: 867 aa, 94 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC028244
• Gene symbol: E2F8
• Gene ID (NCBI): 79733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)

Publikationen

Species	Application	Title
human	WB	Int J Cancer; HOXD-AS1/miR-130a sponge regulates glioma development by targeting E2F8.; Authors - Yinan Chen
human	WB,ChIP	Adv Sci (Weinh); Genetic Aberrations and Interaction of NEK2 and TP53 Accelerate Aggressiveness of Multiple Myeloma; Authors - Xiangling Feng
human	ChIP	Int J Toxicol; Transcription Factor E2F8 Promotes Cisplatin Resistance in Hepatocellular Carcinoma by Regulating DNA Damage via NUSAP1; Authors - Jianqiao Kong
human	WB	Cell Death Discov; Carmofur prevents cell cycle progression by reducing E2F8 transcription in temozolomide-resistant glioblastoma cells; Authors - Cyntanna C Hawkins