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E2F8 Polyklonaler Antikörper

E2F8 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13425-1-PBS

Synonyme

E2F 8, E2F transcription factor 8, E2F-8, Transcription factor E2F8

Filter:
  • Western Blot (WB) analysis of HeLa cells using E2F8 Polyclonal antibody (13425-1-AP)

    WB analysis of HeLa using 13425-1-AP (same clone as 13425-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using E2F8 Polyclonal antibody (13425-1-AP)

    WB analysis of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using E2F8 Polyclonal antibody (13425-1-AP)

    IP experiment of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

    IP result of anti-E2F8 (IP:13425-1-AP, 4ug; Detection:13425-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

13425-1-PBS bindet in WB, IP, Indirect ELISA E2F8 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen E2F8 fusion protein Ag4216
Vollständiger Name E2F transcription factor 8
Berechnetes Molekulargewicht 867 aa, 94 kDa
Beobachtetes Molekulargewicht 105 kDa
GenBank-ZugangsnummerBC028244
Gene symbol E2F8
Gene ID (NCBI) 79733
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)