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EAAT4 Polyklonaler Antikörper

EAAT4 Polyklonal Antikörper für WB, IF-Fro, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12876-1-PBS

Synonyme

SLC1A6, Sodium-dependent glutamate/aspartate transporter

Filter:
  • Western Blot (WB) analysis of A375 cells using EAAT4 Polyclonal antibody (12876-1-AP)

    WB analysis of A375 using 12876-1-AP (same clone as 12876-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12876-1-AP (EAAT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using EAAT4 Polyclonal antibody (12876-1-AP)

    IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using EAAT4 Polyclonal antibody (12876-1-AP)

    IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12876-1-PBS bindet in WB, IF-Fro, Indirect ELISA EAAT4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EAAT4 fusion protein Ag3554
Vollständiger Name solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
Berechnetes Molekulargewicht 61.6 kDa
Beobachtetes Molekulargewicht 60-70 kDa
GenBank-ZugangsnummerBC028721
Gene symbol EAAT4
Gene ID (NCBI) 6511
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excitatory amino acid transporter 4 (EAAT4) is a neuronal glutamate transporter responsible for the reuptake of synaptically released glutamate. The EAAT4 protein is highly enriched in Purkinje cells of the cerebellum, although it is not restricted to these cells (18770868). While the predicted molecular weight of EAAT4 is 62 kDa, considerable heterogeneity in transporter size has been reported in the brain with molecular weights ranging from 50 to 120 kDa as a result of differential glycosylation and/or phosphorylation (24056007). This antibody recognizes 60-70 kDa of EAAT4 in mouse brain and several other lysate.