EAF6 Polyklonaler Antikörper

EAF6 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26465-1-AP

Synonyme

C1orf149, CDABP0189, CENP-28, Chromatin modification-related protein MEAF6, Esa1-associated factor 6 homolog

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  WB analysis of HepG2 using 26465-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26465-1-AP (EAF6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26465-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26465-1-AP (EAF6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26465-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26465-1-AP (EAF6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26465-1-AP

  IP result of anti-EAF6 (IP:26465-1-AP, 4ug; Detection:26465-1-AP 1:500) with HepG2 cells lysate 3480ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26465-1-AP bindet in WB, IP, ELISA EAF6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EAF6 fusion protein Ag23971
• Vollständiger Name: chromosome 1 open reading frame 149
• Beobachtetes Molekulargewicht: 22-28 kDa
• GenBank-Zugangsnummer: BC016328
• Gene symbol: MEAF6
• Gene ID (NCBI): 64769
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Discov; MOZ and Menin-MLL Complexes are Complementary Regulators of Chromatin Association and Transcriptional Output in Gastrointestinal Stromal Tumor.; Authors - Matthew L Hemming
mouse	WB	Cell Death Differ; MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.; Authors - Lifang Hu