EBI3/IL-27B Monoklonaler Antikörper

EBI3/IL-27B Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F3H10

Kat-Nr. : 66354-1-Ig

Synonyme

EBI3, 1F3H10, EBV induced gene 3 protein, EBV-induced gene 3 protein, Epstein Barr virus induced 3

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  WB analysis of human spleen using 66354-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66354-1-Ig (EBI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 66354-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66354-1-Ig (EBI3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, Rattenmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66354-1-Ig bindet in WB, ELISA EBI3/IL-27B und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EBI3/IL-27B fusion protein Ag3051
• Vollständiger Name: Epstein-Barr virus induced 3
• Berechnetes Molekulargewicht: 229 aa, 25 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC015364
• Gene symbol: EBI3
• Gene ID (NCBI): 10148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)