EBP50/NHERF-1 Polyklonaler Antikörper

EBP50/NHERF-1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29771-1-AP

Synonyme

EBP50, NHERF, NHERF 1, NHERF1, SLC9A3R1

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Galerie der Validierungsdaten

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  WB analysis using 29771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 29771-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29771-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 29771-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 29771-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 29771-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HepG2 using 29771-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 (29771-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HepG2-Zellen, Mausnierengewebe, MCF-7-Zellen, MDA-MB-231-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29771-1-AP bindet in WB, IHC, IF, FC, ELISA EBP50/NHERF-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EBP50/NHERF-1 fusion protein Ag30878
• Vollständiger Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: NM_004252
• Gene symbol: SLC9A3R1
• Gene ID (NCBI): 9368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).