EBP50/NHERF-1 Polyklonaler Antikörper

EBP50/NHERF-1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29771-1-PBS

Synonyme

SLC9A3R1, NHERF-1, EBP50

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  WB analysis using 29771-1-AP (same clone as 29771-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29771-1-AP (same clone as 29771-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29771-1-AP (same clone as 29771-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 29771-1-AP (same clone as 29771-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 (29771-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29771-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA EBP50/NHERF-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EBP50/NHERF-1 fusion protein Ag30878
• Vollständiger Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: NM_004252
• Gene symbol: SLC9A3R1/EBP50/NHERF-1
• Gene ID (NCBI): 9368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).