ECH1 Polyklonaler Antikörper

ECH1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11385-1-PBS

Synonyme

HPXEL, EC:5.3.3.-, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

---

• 

  WB analysis of mouse liver using 11385-1-AP (same clone as 11385-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11385-1-AP (same clone as 11385-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11385-1-AP (same clone as 11385-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11385-1-AP (same clone as 11385-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11385-1-AP (same clone as 11385-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 11385-1-AP (same clone as 11385-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 11385-1-AP (same clone as 11385-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 11385-1-AP (same clone as 11385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11385-1-AP (ECH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

11385-1-PBS bindet in WB, IHC, Indirect ELISA ECH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ECH1 fusion protein Ag1931
• Vollständiger Name: enoyl Coenzyme A hydratase 1, peroxisomal
• Berechnetes Molekulargewicht: 328 aa, 36 kDa
• Beobachtetes Molekulargewicht: 30-36 kDa
• GenBank-Zugangsnummer: BC017408
• Gene symbol: ECH1
• Gene ID (NCBI): 1891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Enoyl coenzyme A hydratase 1 (ECH1), is first recog-nized as a key component in mitochondrial fatty acid β-oxidation, and subsequent studies have demonstrated that it regulates multiple pathophysiological processes (PMID:33813878). The MW of ECH1 is 36 kDa,and can be detected as 30-36 kDa.