ECH1 Monoklonaler Antikörper

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ECH1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C9G5

Kat-Nr. : 68139-1-PBS

Synonyme

1C9G5, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, EC:5.3.3.-, HPXEL

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  WB analysis using 68139-1-Ig (same clone as 68139-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

  WB result of ECH1 antibody (68139-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECH1 transfected HepG2 cells. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68139-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ECH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ECH1 fusion protein Ag32661
• Vollständiger Name: enoyl Coenzyme A hydratase 1, peroxisomal
• Berechnetes Molekulargewicht: 328 aa, 36 kDa
• Beobachtetes Molekulargewicht: 33-36 kDa
• GenBank-Zugangsnummer: BC017408
• Gene symbol: ECH1
• Gene ID (NCBI): 1891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.