ECM1 Monoklonaler Antikörper

ECM1 Monoklonal Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

6A12B3

Kat-Nr. : 66023-1-PBS

Synonyme

6A12B3, extracellular matrix protein 1, Secretory component p85

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  WB analysis of MCF-7 using 66023-1-Ig (same clone as 66023-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66023-1-Ig (same clone as 66023-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66023-1-Ig (same clone as 66023-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50074-1

  Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

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  Sample test of MP50074-1

  Serum of eight individual healthy human donors was measured. The human ECM1 concentration of detected samples was determined to be 254.48 ng/mL with a range of 71.72 - 451.84 ng/mL.

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  IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66023-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test ECM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ECM1 fusion protein Ag17955
• Vollständiger Name: extracellular matrix protein 1
• Berechnetes Molekulargewicht: 540 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC023505
• Gene symbol: ECM1
• Gene ID (NCBI): 1893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.