ECM1 Rekombinanter Antikörper

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• KD/KO Validated

ECM1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230321F12

Kat-Nr. : 83067-6-RR

Synonyme

230321F12, extracellular matrix protein 1, Secretory component p85

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  WB analysis of A431 using 83067-6-RR

  WB result of ECM1 antibody (83067-6-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECM1 transfected A431 cells.

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  WB analysis using 83067-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83067-6-RR (ECM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A375 using 83067-6-RR

  1x10^6 A375 cells were intracellularly stained with 0.25 ug Anti-Human ECM1 (83067-6-RR, Clone:230321F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83067-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83067-6-RR against Human ECM1 were performed. The affinity constant is 0.419 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A431-Zellen, L02-Zellen
• Erfolgreiche Detektion in FC (Intra): A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83067-6-RR bindet in WB, FC (Intra), ELISA ECM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ECM1 fusion protein Eg0533
• Vollständiger Name: extracellular matrix protein 1
• Berechnetes Molekulargewicht: 540 aa, 61 kDa
• Beobachtetes Molekulargewicht: 60-80 kDa
• GenBank-Zugangsnummer: BC023505
• Gene symbol: ECM1
• Gene ID (NCBI): 1893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.