ECSIT Polyklonaler Antikörper

ECSIT Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27548-1-PBS

Synonyme

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila)

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  WB analysis using 27548-1-AP (same clone as 27548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27548-1-AP (same clone as 27548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27548-1-PBS bindet in WB, IHC, Indirect ELISA ECSIT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ECSIT fusion protein Ag26877
• Vollständiger Name: ECSIT homolog (Drosophila)
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC008279
• Gene symbol: ECSIT
• Gene ID (NCBI): 51295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).