ECSIT Rekombinanter Antikörper

ECSIT Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240223A7

Kat-Nr. : 83295-1-PBS

Synonyme

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila), 240223A7

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  WB analysis using 83295-1-RR (same clone as 83295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83295-1-RR (ECSIT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00315-1

  Cytometric bead array standard curve of MP00315-1, ECSIT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83295-4-PBS. Detection antibody: 83295-1-PBS. Standard: Ag26898. Range: 3.13-100 ng/mL.

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  Affinity and Kinetic Characterization of 83295-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83295-1-RR against Human ECSIT were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83295-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA ECSIT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ECSIT fusion protein Ag26898
• Vollständiger Name: ECSIT homolog (Drosophila)
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC008279
• Gene symbol: ECSIT
• Gene ID (NCBI): 51295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).