EDAR Polyklonaler Antikörper

EDAR Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18032-1-AP

Synonyme

DL, Downless homolog, Ectodermal dysplasia receptor, ectodysplasin A receptor, ED1R, ED3, ED5, EDA A1 receptor, EDA A1R, EDA1R, EDA3, EDAR, HRM1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 18032-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 18032-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 18032-1-AP

  IP Result of anti-EDAR (IP:18032-1-AP, 4ug; Detection:18032-1-AP 1:500) with A549 cells lysate 2800ug.

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  FC experiment of HepG2 using 18032-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug EDAR antibody (18032-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

18032-1-AP bindet in WB, IP, IHC, IF, FC, ELISA EDAR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EDAR fusion protein Ag12601
• Vollständiger Name: ectodysplasin A receptor
• Berechnetes Molekulargewicht: 448 aa, 49 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC093872
• Gene symbol: EDAR
• Gene ID (NCBI): 10913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.

Publikationen

Species	Application	Title
mouse	IF	Development; Involvement of Wnt, Eda and Shh at defined stages of sweat gland development.; Authors - Chang-Yi Cui
mouse	WB	J Pharm Pharmacol; Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.; Authors - Zhong-San Zeng
mouse	IF,IHC	J Histochem Cytochem; Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice; Authors - Ludan Xing