EDC4 Polyklonaler Antikörper

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• KD/KO Validated

EDC4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 17737-1-AP

Synonyme

Autoantigen Ge 1, Autoantigen RCD 8, EDC4, enhancer of mRNA decapping 4, Ge 1, HEDLS, RCD 8

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17737-1-AP

  WB result of EDC4 antibody (17737-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EDC4 transfected HeLa cells.

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  WB analysis using 17737-1-AP

  HeLa cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 17737-1-AP (EDC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EDC4 antibody (17737-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 17737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EDC4 antibody (17737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 3 publications below

Produktinformation

17737-1-AP bindet in WB, IHC, IF, ELISA EDC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EDC4 fusion protein Ag11784
• Vollständiger Name: enhancer of mRNA decapping 4
• Berechnetes Molekulargewicht: 1401 aa, 152 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC064567
• Gene symbol: EDC4
• Gene ID (NCBI): 23644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.; Authors - Tao Liu
mouse	WB	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	IF	FEBS Lett; Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.; Authors - Dongxu Jiang
mouse	WB	J Cell Biochem; NUSAP1 regulates mouse oocyte meiotic maturation; Authors - Lina Yu
human	IF	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden
mouse	WB,IF	Cell Biosci; QKI degradation in macrophage by RNF6 protects mice from MRSA infection via enhancing PI3K p110β dependent autophagy; Authors - Dongsheng Zhai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (03-01-2022)

RPE1 cells stained for Hoechst (DNA marker, in green), EDC4 (mRNA decapping protein 4 in (P)-bodies, in magenta) and NEDD1 (pericentriolar matrix marker, in yellow). RPE1 cells were fixed in 4%PFA for 15’. Cells were then washed with PBS. Membrane permeabilization was then performed with 0.3% Triton for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

EDC4 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:17737-1-AP)

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