EDC4 Polyklonaler Antikörper

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• KD/KO Validated

EDC4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (1)

Kat-Nr. : 17737-1-AP

Synonyme

Autoantigen Ge-1, Autoantigen RCD-8, Enhancer of mRNA-decapping protein 4, Ge 1, Hedls

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  WB analysis of HeLa using 17737-1-AP

  WB result of EDC4 antibody (17737-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EDC4 transfected HeLa cells.

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  WB analysis using 17737-1-AP

  HeLa cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 17737-1-AP (EDC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EDC4 antibody (17737-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 17737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EDC4 antibody (17737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 9 publications below

Produktinformation

17737-1-AP bindet in WB, IHC, IF/ICC, ELISA EDC4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EDC4 fusion protein Ag11784
• Vollständiger Name: enhancer of mRNA decapping 4
• Berechnetes Molekulargewicht: 1401 aa, 152 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC064567
• Gene symbol: EDC4
• Gene ID (NCBI): 23644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Enhancer of mRNA-decapping protein 4 (EDC4) is a key regulator of mRNA decapping and degradation, playing a crucial role in the mRNA decay pathway. It is involved in the formation of processing bodies (P-bodies) in the cytoplasm, where it interacts with other decapping factors such as DCP1A and DCP2A to facilitate mRNA degradation (PMID: 16364915). EDC4 also interacts with the mammalian target of rapamycin complex 1 (mTORC1) and is involved in the regulation of immune system functions (PMID: 25514416; 25970328). Additionally, EDC4 has been shown to regulate DNA repair processes, with its deficiency leading to genome instability and hypersensitivity to DNA interstrand cross-linking drugs (PMID: 29511213).

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
human	IF	Acta Pharmacol Sin; NSCLC cells sustain phase separation of cytoplasmic membrane-less organelles to protect themselves against cisplatin treatment; Authors - Ning-Ning Li
human	WB	Mol Ther Nucleic Acids; The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.; Authors - Tao Liu
	WB	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	IF	FEBS Lett; Insight into the function of the Golgi membrane protein GOLM1 in cholangiocytes through interactomic analysis; Authors - Meghana Nagaraj

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (03-01-2022)

RPE1 cells stained for Hoechst (DNA marker, in green), EDC4 (mRNA decapping protein 4 in (P)-bodies, in magenta) and NEDD1 (pericentriolar matrix marker, in yellow). RPE1 cells were fixed in 4%PFA for 15’. Cells were then washed with PBS. Membrane permeabilization was then performed with 0.3% Triton for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

EDC4 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:17737-1-AP)