EDG2/LPA1 Polyklonaler Antikörper

EDG2/LPA1 Polyklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20442-1-PBS

Synonyme

LPAR1, edg 2, EDG2, Gpcr26, GPR26

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  WB analysis of Jurkat using 20442-1-AP (same clone as 20442-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of multi-cells/tissue using 20442-1-AP (same clone as 20442-1-PBS)

  Multi-cells/tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20442-1-AP (same clone as 20442-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 20442-1-AP (same clone as 20442-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20442-1-AP (same clone as 20442-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 20442-1-AP (same clone as 20442-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug EDG2 Polyclonal antibody (20442-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20442-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA EDG2/LPA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EDG2/LPA1 fusion protein Ag14253
• Vollständiger Name: lysophosphatidic acid receptor 1
• Berechnetes Molekulargewicht: 364 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41-50 kDa
• GenBank-Zugangsnummer: BC030615
• Gene symbol: EDG2
• Gene ID (NCBI): 1902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EDG2 (also known as LPA1) is a G-protein coupled receptor for lysophosphatidic acid (LPA), a potent motility inducing factor, which is a major component of serum (PMID: 19415462). EDG2 is widely expressed in normal tissue during growth and development. In the context of cancer, several studies have suggested that EDG2 expression in tumors is often similar to that shown in normal tissue (PMID: 17496233).