EDIL3 Rekombinanter Antikörper

EDIL3 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251179F12

Kat-Nr. : 86715-1-RR

Synonyme

DEL1, Developmentally-regulated endothelial cell locus 1 protein, EGF-like repeat and discoidin I-like domain-containing protein 3, Integrin-binding protein DEL1

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  WB analysis using 86715-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86715-1-RR (EDIL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 86715-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EDIL3 antibody (86715-1-RR, Clone: 251179F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HUVEC-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86715-1-RR bindet in WB, IF/ICC, ELISA EDIL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EDIL3 fusion protein Ag3274
• Vollständiger Name: EGF-like repeats and discoidin I-like domains 3
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC030828
• Gene symbol: EDIL3
• Gene ID (NCBI): 10085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGF-like repeats and discoidin I-like domain-containing protein 3 (EDIL3), also named as DEL1 or integrin-biding protein DEL1, is a 52-kDa extracellular matrix protein produced by endothelial cells in embryos (PMID: 9420328). It is composed of three EGF repeats and two discoidin I-like domains. The second EGF repeat contains an RGD motif. EDIL3 promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. It may be involved in vascular remodeling during angiogenesis (PMID: 12840057; 14981004). EDIL3 has also been reported to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion (PMID: 19008446).