EDNRB Polyklonaler Antikörper

EDNRB Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 20964-1-AP

Synonyme

ABCDS, EDNRB, Endothelin B receptor, endothelin receptor type B, ET B, ET BR, ETB, ETBR, ETRB, HSCR, HSCR2

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 20964-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20964-1-AP (EDNRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 20964-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20964-1-AP (EDNRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20964-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20964-1-AP (EDNRB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20964-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20964-1-AP (EDNRB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 20964-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20964-1-AP (EDNRB Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Raji using 20964-1-AP

  1X10^6 Raji cells were stained with 0.2ug EDNRB antibody (20964-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Plazenta-Gewebe, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

20964-1-AP bindet in WB, IHC, IF, FC, ELISA EDNRB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: endothelin receptor type B
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: NM_000115
• Gene symbol: EDNRB
• Gene ID (NCBI): 1910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Nucleic acid ligands act as a PAM and agonist depending on the intrinsic ligand binding state of P2RY2.; Authors - Masaki Takahashi
mouse	WB	Vascul Pharmacol; Tail vein injection of mmLDL upregulates mouse mesenteric artery ETB receptors via activation of the ERK1/2 pathway.; Authors - Yang Liu
mouse	IF,FC	Pigment Cell Melanoma Res; The immunosuppressive role of Edn3 overexpression in the melanoma microenvironment.; Authors - Juliano T Freitas
mouse	WB	BMC Ophthalmol; Functions of retinal astrocytes and Müller cells in mammalian myopia; Authors - Xuhong Zhang
mouse	WB	J Pharm Pharmacol; Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.; Authors - Zhong-San Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jaouhara (Verified Customer) (12-05-2023)

Immunofluorescence analysis of paraffin-fixed murine chondrocytes using anti-EDNRB (20964-1-AP) and an Alexa 647 secondary antibody. Also validated in flow cytometry and immunohistochemistry.

• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry
• Cell Tissue Type: Chondrocytes

EDNRB Antibody Immunohistochemistry,Immunofluorescence,Flow Cytometry validation ( dilution) in Chondrocytes (Cat no:20964-1-AP)

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