EEA1 Monoklonaler Antikörper

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EEA1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E11E4

Kat-Nr. : 68065-1-PBS

Synonyme

1E11E4, Early endosome antigen 1, Endosome-associated protein p162, MST105, MSTP105

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  WB analysis of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

  WB result of EEA1 antibody (68065-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

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  WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68065-1-Ig (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68065-1-Ig (same clone as 68065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68065-1-Ig (EEA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68065-1-Ig (same clone as 68065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68065-1-Ig (EEA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68065-1-Ig (same clone as 68065-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68065-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EEA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEA1 fusion protein Ag28814
• Vollständiger Name: early endosome antigen 1
• Berechnetes Molekulargewicht: 162 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: NM_003566
• Gene symbol: EEA1
• Gene ID (NCBI): 8411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.