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EEA1 Rekombinanter Antikörper

EEA1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C11

Kat-Nr. : 81245-1-PBS

Synonyme

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

Filter:
  • Western Blot (WB) analysis of various lysates using EEA1 Recombinant antibody (81245-1-RR)

    WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81245-1-RR (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EEA1 Recombinant antibody (81245-1-RR)

    WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

    WB result of EEA1 antibody (81245-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81245-1-RR

    Affinity and Kinetic Characterization of 81245-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81245-1-RR against Human EEA1 were performed. The affinity constant is 0.227 nM.

Geprüfte Anwendungen

Produktinformation

81245-1-PBS bindet in WB, Indirect ELISA EEA1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen EEA1 fusion protein Ag28814
Vollständiger Name early endosome antigen 1
Berechnetes Molekulargewicht 162 kDa
Beobachtetes Molekulargewicht 162 kDa
GenBank-ZugangsnummerNM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.