EED Polyklonaler Antikörper

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EED Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16818-1-PBS

Synonyme

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  WB analysis of COLO 320 using 16818-1-AP (same clone as 16818-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16818-1-AP (same clone as 16818-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 16818-1-AP (same clone as 16818-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 16818-1-AP (same clone as 16818-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 16818-1-AP (same clone as 16818-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 16818-1-AP (same clone as 16818-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  IP experiment of PC-3 using 16818-1-AP (same clone as 16818-1-PBS)

  IP result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

  Immunofluorescent analysis of (10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16818-1-AP (same clone as 16818-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EED (16818-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16818-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA EED und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EED fusion protein Ag10358
• Vollständiger Name: embryonic ectoderm development
• Berechnetes Molekulargewicht: 466 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC068995
• Gene symbol: EED
• Gene ID (NCBI): 8726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.