EEF1A1 Monoklonaler Antikörper

EEF1A1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A9F5

Kat-Nr. : 67495-1-PBS

Synonyme

1A9F5, CCS 3, CCS3, EC:3.6.5.-, EEF 1

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  WB analysis using 67495-1-Ig (same clone as 67495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67495-1-Ig (EEF1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

  WB result of EEF1A1 antibody (67495-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67495-1-Ig (same clone as 67495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67495-1-Ig (same clone as 67495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67495-1-Ig (same clone as 67495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67495-1-Ig (same clone as 67495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67495-1-Ig (EEF1A1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EEF1A1 (67495-1-Ig, Clone:1A9F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67495-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA EEF1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF1A1 fusion protein Ag1938
• Vollständiger Name: eukaryotic translation elongation factor 1 alpha 1
• Berechnetes Molekulargewicht: 462 aa, 50 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC014224
• Gene symbol: EEF1A1
• Gene ID (NCBI): 1915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.