EEF1D Monoklonaler Antikörper

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EEF1D Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B1B11

Kat-Nr. : 60085-1-PBS

Synonyme

3B1B11, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

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  WB analysis of Jurkat using 60085-1-Ig (same clone as 60085-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60085-1-Ig (same clone as 60085-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60085-1-Ig (same clone as 60085-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60085-1-Ig (same clone as 60085-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60085-1-Ig (same clone as 60085-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 60085-1-Ig (same clone as 60085-1-PBS)

  IP result of anti-EEF1D (IP:60085-1-Ig, 5ug; Detection:60085-1-Ig 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60085-1-Ig(EEF1D antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60085-1-Ig(EEF1D antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1D antibody (60085-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60085-1-Ig (same clone as 60085-1-PBS)

  Immunofluorescent analysis of Hela cells, using EEF1D antibody 60085-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60085-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 60085-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60085-1-Ig against Human EEF1D were performed. The Affinity Constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

60085-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA EEF1D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF1D fusion protein Ag0983
• Vollständiger Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC007847
• Gene symbol: EEF1D
• Gene ID (NCBI): 1936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )