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EEF1D Monoklonaler Antikörper

EEF1D Monoklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G4C4

Kat-Nr. : 60085-2-PBS

Synonyme

3G4C4, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of Jurkat using 60085-2-Ig (same clone as 60085-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of NIH/3T3 using 60085-2-Ig (same clone as 60085-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of HeLa using 60085-2-Ig (same clone as 60085-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of RAW 264.7 using 60085-2-Ig (same clone as 60085-2-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    IP experiment of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    IP result of anti-EEF1D (IP:60085-2-Ig, 5ug; Detection:60085-2-Ig 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1D Monoclonal antibody (60085-2-Ig)

    IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1D Monoclonal antibody (60085-2-Ig)

    IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 60085-2-Ig

    Affinity and Kinetic Characterization of 60085-2-Ig

    Biolayer interferometry (BLl) kinetic assays of 60085-2-Ig against Human EEF1D were performed. The Affinity Constant is 0.76 nM.

Geprüfte Anwendungen

Produktinformation

60085-2-PBS bindet in WB, IHC, IP, Indirect ELISA EEF1D und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen EEF1D fusion protein Ag0983
Vollständiger Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 35-40 kDa
GenBank-ZugangsnummerBC007847
Gene symbol EEF1D
Gene ID (NCBI) 1936
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )