EEF1D Monoklonaler Antikörper

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• KD/KO Validated

EEF1D Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B1B11

Kat-Nr. : CL488-60085

Synonyme

EF-1-delta, EF1D, EF 1D, EF 1 delta, Antigen NY-CO-4

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  IF Staining of HepG2 using CL488-60085

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-60085 (EEF1D antibody), at dilution of 1:100.

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  IF Staining of HeLa using CL488-60085

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-60085 (EEF1D antibody) at dilution of 1:100.

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  FC experiment of MCF-7 using CL488-60085

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EEF1D (CL488-60085, Clone:3B1B11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL488-60085

  1X10^6 HeLa cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human EEF1D (CL488-60085, Clone:3B1B11) (red), or 1 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60085 bindet in IF/ICC, FC (Intra) EEF1D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF1D fusion protein Ag0983
• Vollständiger Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC007847
• Gene symbol: EEF1D
• Gene ID (NCBI): 1936
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )