EEF1E1 Polyklonaler Antikörper

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• KD/KO Validated

EEF1E1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10805-1-AP

Synonyme

AIMP3, EEF1E1, Elongation factor p18, P18

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Galerie der Validierungsdaten

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  WB analysis using 10805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10805-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10805-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10805-1-AP (EEF1E1 antibody) at dilution of 1:10 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hodengewebe, Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

10805-1-AP bindet in WB, IHC, IF, ELISA EEF1E1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EEF1E1 fusion protein Ag1092
• Vollständiger Name: eukaryotic translation elongation factor 1 epsilon 1
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC005291
• Gene symbol: EEF1E1
• Gene ID (NCBI): 9521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

During the translation processes, aminoacyl-tRNA synthetases (ARSs) ligate specific amino acids to tRNAs. Together with non-enzymatic proteins called ARS-interacting multifunctional proteins (AIMPs), the ARSs form a macromolecular complex [PMID:15286174]. This complex has an essential role in protein synthesis, but also in other biological processes including angiogenesis, apoptosis, and inflammation. [PMID:21789020] AIMP3 is not only associated with the multi-tRNA synthetase complex via its specific interaction with methionyl-tRNA synthetase, but also works as a tumor suppressor by interacting with ATM, the upstream kinase of p53 [PMID:18343821].

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; AIMP3 inhibits cell growth and metastasis of lung adenocarcinoma through activating a miR-96-5p-AIMP3-p53 axis.; Authors - Liting Ding
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
human	WB,IF	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated