EEF1G Monoklonaler Antikörper

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EEF1G Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7C6

Kat-Nr. : 68148-1-PBS

Synonyme

eEF 1B gamma, eEF-1B gamma, EF 1 gamma, EF1G, EF-1-gamma

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  WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68148-1-Ig (same clone as 68148-1-PBS)

  WB result of EEF1G antibody (68148-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1G transfected MCF-7 cells. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68148-1-PBS bindet in WB, IF/ICC, Indirect ELISA EEF1G und zeigt Reaktivität mit Hausschwein, human, Kaninchen

• Getestete Reaktivität: Hausschwein, human, Kaninchen
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF1G fusion protein Ag0218
• Vollständiger Name: eukaryotic translation elongation factor 1 gamma
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC006520
• Gene symbol: EEF1G
• Gene ID (NCBI): 1937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.