EEF2 Monoklonaler Antikörper

EEF2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E4D5

Kat-Nr. : 67550-1-Ig

Synonyme

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

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  WB analysis using 67550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67550-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67550-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67550-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67550-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67550-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67550-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of HepG2 using 67550-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67550-1-Ig (EEF2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67550-1-Ig bindet in WB, IHC, IF/ICC, ELISA EEF2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF2 fusion protein Ag13859
• Vollständiger Name: eukaryotic translation elongation factor 2
• Berechnetes Molekulargewicht: 858 aa, 95 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC126259
• Gene symbol: EEF2
• Gene ID (NCBI): 1938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.