EEFSEC Polyklonaler Antikörper

EEFSEC Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10628-1-PBS

Synonyme

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  WB analysis using 10628-1-AP (same clone as 10628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 10628-1-AP (same clone as 10628-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10628-1-AP (same clone as 10628-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10628-1-PBS bindet in WB, IHC, Indirect ELISA EEFSEC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EEFSEC fusion protein Ag0980
• Vollständiger Name: eukaryotic elongation factor, selenocysteine-tRNA-specific
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC007933
• Gene symbol: EEFSEC
• Gene ID (NCBI): 60678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon.