EFHD2 Rekombinanter Antikörper

EFHD2 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240185H5

Kat-Nr. : 83264-5-PBS

Synonyme

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185H5

---

• 

  WB analysis using 83264-5-RR (same clone as 83264-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83264-5-RR (EFHD2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83264-5-RR (same clone as 83264-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using EFHD2 antibody (83264-5-RR, Clone: 240185H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83264-5-RR (same clone as 83264-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-5-RR, Clone:240185H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83264-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83264-5-RR against Human EFHD2 were performed. The affinity constant is 1.42 nM.

---

Geprüfte Anwendungen

Produktinformation

83264-5-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA EFHD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EFHD2 fusion protein Ag35084
• Vollständiger Name: EF-hand domain family, member D2
• Berechnetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC023611
• Gene symbol: EFHD2
• Gene ID (NCBI): 79180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.