EFHD2 Rekombinanter Antikörper

EFHD2 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240185A8

Kat-Nr. : 83264-6-PBS

Synonyme

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2, 240185A8

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  WB analysis using 83264-6-RR (same clone as 83264-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83264-6-RR (EFHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83264-6-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83264-6-RR (same clone as 83264-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-6-RR, Clone:240185A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83264-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83264-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83264-6-RR against Human EFHD2 were performed. The affinity constant is 4.63 nM.

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Geprüfte Anwendungen

Produktinformation

83264-6-PBS bindet in WB, FC (Intra), ELISA EFHD2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EFHD2 fusion protein Ag35084
• Vollständiger Name: EF-hand domain family, member D2
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC023611
• Gene symbol: EFHD2
• Gene ID (NCBI): 79180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EFHD2 also named Swiprosin-1, is a Ca2+ binding actin protein. The expression of EFHD2 is upregulated during the activation of immune cells, epithelial and endothelial cells. The expression of EFHD2 is regulated by diverse signaling pathways that are contingent upon the specific type of cells.